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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Second Messenger Nucleotide Messenger GTP

Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)

Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)
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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15593] to TAGAP - BSA and Azide free
  • Suitable for: IP, Flow Cyt (Intra), WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-TAGAP antibody [EPR15593] - BSA and Azide free
    See all TAGAP primary antibodies
  • Description

    Rabbit monoclonal [EPR15593] to TAGAP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Flow Cyt (Intra), WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250938 is the carrier-free version of ab187664.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15593
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • GTP
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other

Images

  • Flow Cytometry - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)
    Flow Cytometry - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)
    This data was developed using ab187664, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TAGAP with ab187664 at 1/160 dilution (pink) compared to a Rabbit monoclonal IgG Isotype control (green). Goat anti rabbit IgG (FITC) was used as the secondary antibody at 1/150 dilution.
  • Western blot - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)
    Western blot - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)
    All lanes : Anti-TAGAP antibody [EPR15593] (ab187664) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 81 kDa
    Observed band size: 61,81 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab187664, the same antibody clone in a different buffer formulation.

    Based on sequence analysis, ab187664 recognizes three isoforms with the predicted MWs of 81kDa, 61kDa and 57kDa, respectively.

  • Immunoprecipitation - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)
    Immunoprecipitation - Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)
    This data was developed using ab187664, the same antibody clone in a different buffer formulation.Western blot analysis of TAGAP in A549 cell lysate immunoprecipitated with ab187664 at 1/50 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
  • Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)
    Anti-TAGAP antibody [EPR15593] - BSA and Azide free (ab250938)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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