Anti-6X His tag® antibody [AD1.1.10] (ab15149)
Key features and details
- Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, IHC-Fr, IP, WB
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-6X His tag® antibody [AD1.1.10]
See all 6X His tag® primary antibodies -
Description
Mouse monoclonal [AD1.1.10] to 6X His tag® -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Fr, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
PAX6 transcription factor linked to histidine tag.
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.
The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.58% Sodium chloride, 95% Sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Tissue cuture supernatant purified by Protein G affinity chromatography. -
Primary antibody notes
ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. -
Clonality
Monoclonal -
Clone number
AD1.1.10 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas