Recombinant human CD73 protein (Active) (Biotin) (ab271447)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant human CD73 protein (Active) (Biotin)
See all CD73 proteins and peptides -
Biological activity
Specific activity >7000 pmol/min/μg.
Assay was done in 50 μL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 μM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHT DEMFWNHVSM CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI K -
Predicted molecular weight
61 kDa including tags -
Amino acids
27 to 547 -
Tags
His tag C-Terminus , Avi tag C-Terminus -
Additional sequence information
Genbank: NM_002526
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Conjugation
Biotin
Preparation and Storage
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Alternative names
- 5' NT
- 5' nucleotidase (CD73)
- 5' nucleotidase precursor
see all -
Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
Involvement in disease
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
Sequence similarities
Belongs to the 5'-nucleotidase family. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human CD73 protein (Active) (Biotin) (ab271447)
Functional analysis of ab271447.
Specific activity >7000 pmol/min/μg.
Assay was done in 50 μL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 μM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
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SDS-PAGE - Recombinant Human CD73 protein (Tagged) (Biotin) (ab271447)SDS-PAGE analysis of ab271447.