Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)

Key features and details

  • Mouse monoclonal [9E11] to Myc tag - BSA and Azide free
  • Suitable for: Flow Cyt, IP, ChIP, IHC-P, WB, IHC-Fr
  • Reacts with: Chicken, Human, Saccharomyces cerevisiae
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Myc tag antibody [9E11] - BSA and Azide free
    See all Myc tag primary antibodies

  • Description

    Mouse monoclonal [9E11] to Myc tag - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, IP, ChIP, IHC-P, WB, IHC-Frmore details

  • Species reactivity

    Reacts with: Chicken, Human, Saccharomyces cerevisiae

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP:CHO overexpressing Stra8 whole cell lysate. Flow Cyt: HL60 cells. WB: Recombinant Human c-Myc protein.

  • General notes

    ab264433 is the PBS only version of ab56.

    This antibody clone is manufactured by Abcam.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)
    Immunoprecipitation - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)

    This image was produced using the same antibody clone in a different formulation ab56.

    Myc tag was immunoprecipitated using 0.5mg CHO overexpressing Stra8 whole cell lysate, 5µg of Mouse monoclonal to Myc tag and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, CHO overexpressing Stra8 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 49KDa; Myc tag

  • Flow Cytometry - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)
    Flow Cytometry - Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)

    This image was produced using the same antibody clone in a different formulation ab56.

    Overlay histogram showing HL60 cells stained with ab56 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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