Anti-NAT10 antibody (ab235048)
Key features and details
- Rabbit polyclonal to NAT10
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NAT10 antibody
See all NAT10 primary antibodies -
Description
Rabbit polyclonal to NAT10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Recombinant fragment corresponding to Human NAT10 aa 900 to the C-terminus.
Database link: Q9H0A0 -
Positive control
- WB: HeLa cell lysate. IHC-P: Human pancreatic cancer and brain tissue.
-
General notes
This product was previously labelled as ALP.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
>95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody (ab235048)
Paraffin-embedded human brain tissue stained for NAT10 using ab235048 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
-
Western blot - Anti-NAT10 antibody (ab235048)Anti-NAT10 antibody (ab235048) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody (ab235048)Paraffin-embedded human pancreatic cancer tissue stained for NAT10 using ab235048 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
