Mouse Nidogen-1 ELISA Kit (Entactin) (ab270203)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 44.15 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Nidogen-1 ELISA Kit (Entactin)
See all Entactin/NID kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.4% Inter-assay Sample n Mean SD CV% Serum 3 6.5% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
44.15 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 107 105% - 110% Serum 89 81% - 103% Cell culture media 97 96% - 98% Hep Plasma 92 87% - 100% EDTA Plasma 93 89% - 97% Cit plasma 85 83% - 87% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Nidogen-1 ELISA Kit (Entactin) (ab270203) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nidogen-1 (Entactin) protein in cell culture media, cell culture supernatant, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Nidogen-1 (Entactin) with 44.15 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Nidogen-1 is a glycoprotein found in the basement membrane. Nidogen-1 is a part of a network of proteins within the cell and is thought to be involved in cell-extracellular matrix interactions. Nidogen-1 is a ubiquitous and abundant protein, in contrast to nidogen-2 which has distinct expression patterns. Mice lacking both nidogens die shortly after birth due to lung and heart problems.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Nidogen-1 (Entactin) Capture Antibody 1 x 600µl 10X Mouse Nidogen-1 (Entactin) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse Nidogen-1 (Entactin) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. -
Sequence similarities
Contains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Alternative names
- Enactin
- Ent
- Entactin
see all -
Database links
- Entrez Gene: 18073 Mouse
- SwissProt: P10493 Mouse
- Unigene: 4691 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Nidogen-1 standard curve in Sample Diluent NS.The Nidogen-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Nidogen-1 in mouse serum and plasma samples.The concentrations of Nidogen-1 were measured in duplicates, interpolated from the Nidogen-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 2.5%, plasma (heparin) 2.5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nidogen-1 concentration was determined to be 59.56 ng/mL in serum, 165.19 ng/mL in plasma (citrate), 199.81 ng/ml in plasma (heparin), and 167.56 ng/ml in plasma (EDTA).
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Interpolated concentrations of native Nidogen-1 in mouse heart tissue supernatant samples.The concentrations of Nidogen-1 were measured in duplicates, interpolated from the Nidogen-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: heart supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nidogen-1 concentration was determined to be 27.66 ng/mL in heart supernatant.
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Sandwich ELISA - Mouse Nidogen-1 ELISA Kit (Entactin) (ab270203)To learn more about the advantages of recombinant antibodies see here.
