Mouse Nidogen-1 Antibody Pair - BSA and Azide free (Entactin) (ab253303)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse Nidogen-1 Antibody Pair - BSA and Azide free (Entactin)
See all Entactin/NID kits -
Assay type
ELISA set -
Range
78.125 pg/ml - 5000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse Nidogen-1 (Entactin). BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab270203), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse Nidogen-1 Capture Antibody (unconjugated) 1 x 100µg Mouse Nidogen-1 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. -
Sequence similarities
Contains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Alternative names
- Enactin
- Ent
- Entactin
see all -
Database links
- Entrez Gene: 18073 Mouse
- SwissProt: P10493 Mouse
- Unigene: 4691 Mouse
Images
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Sandwich ELISA - Mouse Nidogen-1 Antibody Pair - BSA and Azide free (Entactin) (ab253303)To learn more about the advantages of recombinant antibodies see here.
