Human IgG ELISA kit - 30 minutes (ab273151)
Key features and details
- Sensitivity: 4 ng/ml
- Range: 16 ng/ml - 1000 ng/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
Overview
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Product name
Human IgG ELISA kit - 30 minutes
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A 1/100 10 6.8µg/ml 10.12 % B 1/100 10 13.4µg/ml 6.13 % C 1/100 10 13.9µg/ml 5.71 % D 1/100 10 18.8µg/ml 5.19 % E 1/100 10 23.5µg/ml 6.5 % F 1/100 10 26.9µg/ml 5.42 % G 1/100 10 32.7µg/ml 8.84 % Inter-assay Sample n Mean SD CV% H 1/250 30 7 % H 1/500 30 4.6 % H 1/1000 30 2.4 % -
Sample type
Cell culture supernatant, Serum -
Assay type
Quantitative -
Sensitivity
4 ng/ml -
Range
16 ng/ml - 1000 ng/ml -
Assay duration
One step assay -
Product overview
The Human IgG quantification Kit (ab273151) provides a rapid and easy method (one step ELISA) for the quantitative determination of human IgG in cell culture supernatant and serum. The kit includes ready-to-use reagents necessary to analyze 89 samples in less than 30 minutes.
A polyclonal antibody specific to the gamma heavy chain of human IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and human IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-human IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of human IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10 x 96 tests Detection antibody 1 x 12ml 1 x 120ml Human IgG standard 0.0 ng/ml 1 x 0.3ml 1 x 1ml Human IgG standard 1000 ng/ml 1 x 0.3ml 1 x 1ml Human IgG standard 125 ng/ml 1 x 0.3ml 1 x 1ml Human IgG standard 16 ng/ml 1 x 0.3ml 1 x 1ml Human IgG standard 250 ng/ml 1 x 0.3ml 1 x 1ml Human IgG standard 500 ng/ml 1 x 0.3ml 1 x 1ml Human IgG standard 63 ng/ml 1 x 0.3ml 1 x 1ml Pre-coated microwells strips 1 x 96 tests 10 x 96 tests Sample diluent 1 x 30ml 1 x 500ml Stop solution 1 x 12ml 1 x 120ml TMB Substrate 1 x 12ml 1 x 120ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
Images
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Human Immunoglobulin G Quantification Standard curve
Example standard curve.
