Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18841-99] to JNK1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free
    See all JNK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18841-99] to JNK1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251542 is the carrier-free version of ab213521. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251542 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    Lane 1 : Human JNK1 full length recombinant protein
    Lane 2 : Human JNK2 full length recombinant protein
    Lane 3 : Human JNK3 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    This data was developed using ab213521, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lanes 2-3: 3 minutes.

    Human JNK1 full length recombinant protein contain aa1-427 with a His-Tag®. Human JNK2 full length recombinant protein contain aa1-424 with a His-Tag®. Human JNK3 full length recombinant protein contain aa1-464 with a His-Tag®.

  • Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46-54 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab213521, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11313405).

  • Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46-54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab213521, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight bserved is consistent with what has been described in the literature (PMID: 11313405).

  • Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat heart tissue lysate
    Lane 6 : Rat kidney tissue lysate
    Lane 7 : Rat spleen tissue lysate
    Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 11 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46-54 kDa why is the actual band size different from the predicted?



    This data was developed using ab213521, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-7: 3 minutes; Lane 8-11: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11313405).

  • Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

    All lanes : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46-54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    This data was developed using ab213521, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    Immunoprecipitation - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    This data was developed using ab213521, the same antibody clone in a different buffer formulation.JNK1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab213521 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab213521 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Lane 1: HeLa whole cell lysate, 10ug (Input). Lane 2: ab213521 IP in HeLa whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab213521 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)
    Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (ab251542)

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