Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free (ab237037)
![Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free (ab237037)](https://www.abcam.com/ps/products/237/ab237037/Images/ab237037-314439-anti-il-1-alpha-antibody-epr19904-247-flow-cytometry.jpg "Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free (ab237037)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19904-247] to IL-1 alpha - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free
See all IL-1 alpha primary antibodies -
Description
Rabbit monoclonal [EPR19904-247] to IL-1 alpha - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human IL-1 alpha recombinant protein (aa113-271); THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, cell lysate. Flow Cyt: THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 µg/ml lipopolysaccharide (LPS) for 24 hours.
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General notes
Ab237037 is the carrier-free version of ab206410. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab237037 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19904-247 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free (ab237037)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) cell line treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 μg/ml lipopolysaccharide (LPS) for 24 hours (red) or an untreated control (green) labeling IL-1 alpha with ab206410 at 1/60 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206410).
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![Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free (ab237037)](https://www.abcam.com/ps/products/237/ab237037/Images/ab237037-2-benefits-of-recombinant-antibodies.png)
Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free (ab237037)
