Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)
![Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)](https://www.abcam.com/ps/products/250/ab250234/Images/ab250234-414737-recombinant-anti-tmub1-antibody-epr14066-western-blot-hela.jpg "Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14066] to TMUB1 - BSA and Azide free
- Suitable for: IHC-P, ICC, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TMUB1 antibody [EPR14066] - BSA and Azide free
See all TMUB1 primary antibodies -
Description
Rabbit monoclonal [EPR14066] to TMUB1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver tissue lysate, Human cerebellum tissue lysate, HeLa cells, HepG2 cells, Human liver tissue.
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General notes
Ab250234 is the carrier-free version of ab180586. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250234 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14066 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/10000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab180586).
Lanes 1- 3: Merged signal (red and green). Green - ab180586 observed at 27 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab180586 was shown to react with TMUB1 in wild-type HeLa (Human epithelial line from cervix adenocarcinoma) cells in western blot. Loss of signal was observed when knockout cell line ab265852 (knockout cell lysate ab258237) was used. Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) and TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180586 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)](https://www.abcam.com/ps/products/250/ab250234/Images/ab250234-4-anti-tmub1-antibody-epr14066-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234) -
![Immunocytochemistry - Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)](https://www.abcam.com/ps/products/250/ab250234/Images/ab250234-3-anti-tmub1-antibody-epr14066-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)](https://www.abcam.com/ps/products/250/ab250234/Images/ab250234-2-anti-tmub1-antibody-epr14066-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)This data was developed using ab180586, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TMUB1 with ab180586 at 1/100. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Western blot - Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)](https://www.abcam.com/ps/products/250/ab250234/Images/ab250234-399279-anti-tmub1-antibody-epr14066-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/50000 dilution
Lane 1 : Human fetal liver tissue lysate at 20 µg
Lane 2 : Human cerebellum tissue lysate at 20 µg
Lane 3 : HepG2 cell line lysate lysate at 20 µg
Lane 4 : HeLa cell line lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa (possible cleavage fragment)This data was developed using ab180586, the same antibody clone in a different buffer formulation.
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![Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)](https://www.abcam.com/ps/products/250/ab250234/Images/ab250234-6-benefits-of-recombinant-antibodies.png)
Anti-TMUB1 antibody [EPR14066] - BSA and Azide free (ab250234)