Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11065(2)] to N myc interactor/NMI - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free
    See all N myc interactor/NMI primary antibodies

  • Description

    Rabbit monoclonal [EPR11065(2)] to N myc interactor/NMI - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, A549, K562 and HepG2 cell lysates. IHC-P: Human tonsil tissue. ICC: HeLa cells.

  • General notes

    ab250698 is the carrier-free version of ab183724 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250698 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as N myc interactor

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    Western blot - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    All lanes : Anti-N myc interactor/NMI antibody [EPR11065(2)] (ab183724) at 1/1000 dilution

    Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : NMI knockout A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab183724, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab183724 observed at 39 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab183724 Anti-N myc interactor/NMI antibody [EPR11065(2)]  was shown to specifically react with N myc interactor/NMI in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267013 (knockout cell lysate ab258077) was used. Wild-type and N myc interactor/NMI knockout samples were subjected to SDS-PAGE. ab183724 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    This data was developed using ab183724, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling N myc interactor/NMI with ab183724 at 1/50 dilution. The slide is counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    Western blot - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)

    This data was developed using ab183724, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: N myc interactor/NMI knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: K562 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab183724 observed at 39 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab183724 was shown to specifically react with N myc interactor/NMI when N myc interactor/NMI knockout samples were used. Wild-type and N myc interactor/NMI knockout samples were subjected to SDS-PAGE. ab183724 and ab18058 (loading control to Vinculin) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    Western blot - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    All lanes : Anti-N myc interactor/NMI antibody [EPR11065(2)] (ab183724) at 1/20000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa



    This data was developed using ab183724, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    Immunocytochemistry - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    This data was developed using ab183724, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of acetone-fixed HeLa cells labeling N myc interactor/NMI with ab183724 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/200 dilution was used as the secondary antibody (green). The slide on the right is stained with Dapi (blue).
  • Immunoprecipitation - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    Immunoprecipitation - Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    This data was developed using ab183724, the same antibody clone in a different buffer formulation.Western blot analysis of K562 cell lysate precipitated with ab183724 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution was then used. The blocking buffer and dilution buffer was 5% NFDM/TBST.
  • Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)
    Anti-N myc interactor/NMI antibody [EPR11065(2)] - BSA and Azide free (ab250698)

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