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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)

Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13520(B)] to RNA Helicase A - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free
    See all RNA Helicase A primary antibodies
  • Description

    Rabbit monoclonal [EPR13520(B)] to RNA Helicase A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250189 is the carrier-free version of ab180189 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250189 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13520(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Images

  • Western blot - Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)
    Western blot - Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)
    All lanes : Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189) at 1/1000 dilution

    Lane 1 : BxPC-3 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 141 kDa



    This data was developed using ab180189, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)
    Immunocytochemistry - Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)
    This data was developed using ab180189, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells labeling RNA Helicase A with ab180189 at 1/100.
  • Flow Cytometry - Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)
    Flow Cytometry - Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)
    This data was developed using ab180189, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of permeabilized Jurkat cells labeling RNA Helicase A with ab180189 at 1/10 (red) or a rabbit IgG negative (green).
  • Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)
    Anti-RNA Helicase A antibody [EPR13520(B)] - BSA and Azide free (ab250189)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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