Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Thursday March 04, 2021

Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19904-247] to IL-1 alpha
Suitable for: WB, Flow Cyt
Reacts with: Human

Product name

Anti-IL-1 alpha antibody [EPR19904-247]
See all IL-1 alpha primary antibodies
Description

Rabbit monoclonal [EPR19904-247] to IL-1 alpha
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human IL-1 alpha recombinant protein (aa113-271); THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, cell lysate. Flow Cyt: THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 µg/ml lipopolysaccharide (LPS) for 24 hours.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19904-247
Isotype

IgG
Research areas

Flow Cytometry - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) cell line treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 μg/ml lipopolysaccharide (LPS) for 24 hours (red) or an untreated control (green) labeling IL-1 alpha with ab206410 at 1/60 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)

All lanes : Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution

Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1, treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 31 kDa
Observed band size: 15,20,34 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Expression of IL-1 alpha can be induced by LPS in macrophages. The molecular masses observed are consistent with the literature (PMID: 9785472; PMID: 1868253; PMID:19104081).
Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)

Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution + Human IL-1 alpha recombinant protein (aa113-271) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 31 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)

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