Anti-HLA G antibody [5A6G7] (ab76869)
![Anti-HLA G antibody [5A6G7] (ab76869)](https://www.abcam.com/ps/products/76/ab76869/Images/ab76869-92389-anti-hla-g-antibody-5a6g7-western-blot.jpg "Anti-HLA G antibody [5A6G7] (ab76869)")
Key features and details
- Mouse monoclonal [5A6G7] to HLA G
- Suitable for: WB
- Isotype: IgG1
Overview
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Product name
Anti-HLA G antibody [5A6G7]
See all HLA G primary antibodies -
Description
Mouse monoclonal [5A6G7] to HLA G -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Immunogen
Other Immunogen Type corresponding to HLA G. C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 protein coupled to ovalbumin
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Positive control
- WB: M8 cell line transfected with HLA-G5 and M8 cell line transfected with HLA-G6.
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General notes
ab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Primary antibody notes
ab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. -
Clonality
Monoclonal -
Clone number
5A6G7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-HLA G antibody [5A6G7] (ab76869)All lanes : Anti-HLA G antibody [5A6G7] (ab76869)
Lane 1 : M8 cell line transfected with empty vector
Lane 2 : M8 cell line transfected with HLA G1
Lane 3 : M8 cell line transfected with HLA G5
Lane 4 : M8 cell line transfected with HLA G6
Predicted band size: 38 kDa
Observed band size: 30,38 kDa why is the actual band size different from the predicted?
