Anti-HLA G antibody [G233] (ab52454)
![Anti-HLA G antibody [G233] (ab52454)](https://www.abcam.com/ps/products/52/ab52454/Images/ab52454-64562-anti-hla-g-antibody-g233-immunofluorescence.jpg "Anti-HLA G antibody [G233] (ab52454)")
Key features and details
- Mouse monoclonal [G233] to HLA G
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-HLA G antibody [G233]
See all HLA G primary antibodies -
Description
Mouse monoclonal [G233] to HLA G -
Host species
Mouse -
Specificity
ab52454 recognises several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). It does not cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F). -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human HLA G. Murine L cells transfected with both human beta2-microglobulin and HLA-G
-
Positive control
- Jeg3 cell line.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
G233 -
Isotype
IgG2a -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [G233] (ab52454)ICC/IF image of ab52454 stained Jeg3 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52454, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
