Anti-HLA G antibody [4H84] (ab52455)
![Anti-HLA G antibody [4H84] (ab52455)](https://www.abcam.com/ps/products/52/ab52455/Images/ab52455-481939-antihla-g-antibody-4h84-western-blot.JPG "Anti-HLA G antibody [4H84] (ab52455)")
Key features and details
- Mouse monoclonal [4H84] to HLA G
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HLA G antibody [4H84]
See all HLA G primary antibodies -
Description
Mouse monoclonal [4H84] to HLA G -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human HLA G aa 61-83.
Sequence:DSDSACPRMEPRAPWVEQEGPEY
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Positive control
- WB: JEG-3 cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
4H84 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-HLA G antibody [4H84] (ab52455)All lanes : Anti-HLA G antibody [4H84] (ab52455) at 2 µg/ml
Lane 1 : JEG-3 cell line under reducing conditions.
Lane 2 : LNCaP cell line under reducing conditions.
Lane 3 : JEG-3 cell line under non-reducing conditions.
Lane 4 : LNCaP cell line under non-reducing conditions.
Secondary
All lanes : IRDye800-conjugated anti-mouse
Predicted band size: 38 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Western blotting analysis of human HLA-G using ab52455 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions.
Nitrocellulose membrane was probed with 2 µg/ml of ab52455 followed by IRDye800-conjugated anti-mouse secondary antibody.
