Anti-HLA G antibody [MEM-G/1] (ab7759)
![Anti-HLA G antibody [MEM-G/1] (ab7759)](https://www.abcam.com/ps/products/7/ab7759/Images/ab7759-454758-anti-hla-g-antibody-mem-g1-immunohistochemistry-placenta-human.jpg "Anti-HLA G antibody [MEM-G/1] (ab7759)")
Key features and details
- Mouse monoclonal [MEM-G/1] to HLA G
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HLA G antibody [MEM-G/1]
See all HLA G primary antibodies -
Description
Mouse monoclonal [MEM-G/1] to HLA G -
Host species
Mouse -
Specificity
The antibody reacts with denaturated HLA-G heavy chain. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length denatured heavy chain (Human).
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Positive control
- IHC-P: First trimester placenta and pulmonary diseases. WB: ab29745; HeLa and JEG-3 cell lysates.
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General notes
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-G/1 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
Immunohistochemistry staining with ab7759 of first trimester placenta (paraffin-embedded sections).
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![Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)](https://www.abcam.com/ps/products/7/ab7759/Images/ab7759-13067-anti-hla-g-antibody-mem-g-1-western-blot.jpg)
Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 10 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 38.2 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)](https://www.abcam.com/ps/products/7/ab7759/Images/ab7759_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)The antibody ab7759 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.
