Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GFP antibody [E385] - BSA and Azide free (ab239807)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E385] to GFP - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Species independent

Overview

  • Product name

    Anti-GFP antibody [E385] - BSA and Azide free
    See all GFP primary antibodies

  • Description

    Rabbit monoclonal [E385] to GFP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Species independent
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Pure GFP protein, or cells known to overexpress GFP.

  • General notes

    ab239807 is the carrier-free version of ab32146 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239807 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    On the basis of low sequence homology, ab32146 is predicted to show no or limited cross-reactivity to RFP, YFP, BFP, and CFP.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GFP antibody [E385] - BSA and Azide free (ab239807)
    Western blot - Anti-GFP antibody [E385] - BSA and Azide free (ab239807)
    Lane 1 : Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution (purified)
    Lanes 2-4 : Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution

    Lane 1 : HeLa whole cell lysate (negative control)
    Lane 2 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.001 µg
    Lane 3 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.002 µg
    Lane 4 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.0025 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    This data was developed using ab32146, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-GFP antibody [E385] - BSA and Azide free (ab239807)
    Western blot - Anti-GFP antibody [E385] - BSA and Azide free (ab239807)
    All lanes : Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution (unpurified)

    Lane 1 : HeLa cells lysate (negative control)
    Lane 2 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.1ng
    Lane 3 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.5ng
    Lane 4 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 1ng
    Lane 5 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2ng
    Lane 6 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2.5ng
    Lane 7 : Proprietary tag-GFP 0.1mg/ml (positive control)

    Predicted band size: 27 kDa



    This data was developed using ab32146, the same antibody clone in a different buffer formulation.

     

  • Anti-GFP antibody [E385] - BSA and Azide free (ab239807)
    Anti-GFP antibody [E385] - BSA and Azide free (ab239807)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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