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Signal Transduction Antibodies gfp

Anti-GFP antibody [E385] (ab32146)

Price and availability

351 792 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-GFP antibody [E385] (ab32146)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E385] to GFP
  • Suitable for: WB
  • Reacts with: Species independent

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Overview

  • Product name

    Anti-GFP antibody [E385]
    See all GFP primary antibodies
  • Description

    Rabbit monoclonal [E385] to GFP
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for GFP and GFP fusion proteins.
  • Tested Applications & Species

    Application Species
    WB
    Species independent
    See all applications and species data
  • Immunogen

    within Aequorea victoria GFP aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P42212

  • Positive control

    • Pure GFP protein, or cells known to overexpress GFP.
  • General notes

    On the basis of low sequence homology, ab32146 is predicted to show no or limited cross-reactivity to RFP and BFP.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.02 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E385
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

Images

  • Western blot - Anti-GFP antibody [E385] (ab32146)
    Western blot - Anti-GFP antibody [E385] (ab32146)
    All lanes : Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution (purified)

    Lane 1 : HeLa whole cell lysate (negative control)
    Lane 2 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.001 µg
    Lane 3 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.002 µg
    Lane 4 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.0025 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-GFP antibody [E385] (ab32146)
    Western blot - Anti-GFP antibody [E385] (ab32146)
    All lanes : Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution (unpurified)

    Lane 1 : HeLa cells lysate (negative control)
    Lane 2 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.1ng
    Lane 3 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.5ng
    Lane 4 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 1ng
    Lane 5 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2ng
    Lane 6 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2.5ng
    Lane 7 : Proprietary tag-GFP 0.1mg/ml (positive control)
  • Anti-GFP antibody [E385] (ab32146)
    Anti-GFP antibody [E385] (ab32146)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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