Sepharose Anti-GFP antibody (ab69314)
Key features and details
- Sepharose Rabbit polyclonal to GFP
- Suitable for: IP
- Reacts with: Species independent
- Conjugation: Sepharose
- Isotype: IgG
Overview
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Product name
Sepharose Anti-GFP antibody
See all GFP primary antibodies -
Description
Sepharose Rabbit polyclonal to GFP -
Host species
Rabbit -
Conjugation
Sepharose -
Specificity
This product is a suspension of affinity purified anti-GFP antibody (ab6556) covalently linked to sepharose beads. It is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP, and EGFP. The unit sold contains 25µg of affinity purified rabbit anti-GFP IgG cross-linked to 125 µl sepharose beads in a total volume of 250 µl buffer. The product is supplied as a 50% slurry to facilitate pipetting. Pipetting the slurry is facilitated when pipet tips are blunted by cutting 3-5mm off from the tip. -
Tested Applications & Species
See all applications and species dataApplication Species IP Species independent
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Immunogen
Recombinant full length protein corresponding to GFP.
Database link: P42212 -
Positive control
- Pure GFP protein, or cells known to overexpress GFP.
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General notes
This product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Sepharose Anti-GFP antibody (ab69314)Lane 1 & 3: IP from COS 7 cells transfected with EGFP.N-Ras (48 kDa)
Lane 2 & 4: IP from untransfected COS 7 cells
Lane 1 & 2: IP using 15 ul of rabbit anti-GFP conjugated to sepharose beads (0.5 mg IgG per ml of beads)
Lane 3 & 4: IP using 15 ul of goat anti-GFP conjugated to sepharose beads (1 mg IgG per ml of beads)
Immunoprecipitation: COS 7 cell lysates containing 100 ug of total protein in 200 ul of 0.1% SDS-RIPA buffer with addition of complete protease inhibitor were used for each immunoprecipitation. Cell lysates were incubated with anti-GFP sepharose beads for 2 hours at 4oC with rocking. Beads were washed. Proteins were eluted with 1% SDS 50 mM HEPES (pH 7.4) at 80oC (15 min). Half of the IP sample was loaded on each lane of 10 % SDS PAGE gel and gel was processed for Western blotting/ECL.
Western blot: Primary antibody: monoclonal mouse anti-GFP (ab291) at 0.2 ug/ml in 5% non-fat milk/TBS-T; 1 hour incubation at room
