Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GFP antibody [EPR14104-89] (ab183735)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14104-89] to GFP
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Species independent

Overview

  • Product name

    Anti-GFP antibody [EPR14104-89]
    See all GFP primary antibodies

  • Description

    Rabbit monoclonal [EPR14104-89] to GFP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Species independent
    ICC/IF
    Species independent
    IHC-P
    Species independent
    WB
    Species independent
    See all applications and species data

  • Immunogen

    Full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • GFP transfected 293 cell lysate; GFP transgenic mouse colon tissue; GFP transgenic mouse liver tissue; GFP transfected 293 cells.

  • General notes

    On the basis of low sequence homology, ab183735 is predicted to show no or limited cross-reactivity to RFP and BFP.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 8.82 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14104-89

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] (ab183735)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] (ab183735)

    Immunohistochemical analysis of paraffin-embedded GFP transgenic mouse colon tissue (left) and normal mouse colon tissue (right) labeling GFP with ab183735 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GFP antibody [EPR14104-89] (ab183735)
    Western blot - Anti-GFP antibody [EPR14104-89] (ab183735)
    All lanes : Anti-GFP antibody [EPR14104-89] (ab183735) at 1/10000 dilution

    Lane 1 : GFP transfected 293 cell lysate
    Lane 2 : Non-transfected 293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] (ab183735)
    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] (ab183735)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed GFP transfected 293 cells labeling GFP with ab183735 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] (ab183735)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] (ab183735)

    Immunohistochemical analysis of paraffin-embedded GFP transgenic mouse liver tissue (left) and normal mouse liver tissue (right) labeling GFP with ab183735 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-GFP antibody [EPR14104-89] (ab183735)
    Flow Cytometry - Anti-GFP antibody [EPR14104-89] (ab183735)

    Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) transfected with GFP cells labeling GFP with unpurified ab183735 at 1/200 dilution (10ug/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.

  • Anti-GFP antibody [EPR14104-89] (ab183735)
    Anti-GFP antibody [EPR14104-89] (ab183735)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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