Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-GFP antibody [E385] (ab190584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [E385] to GFP
  • Suitable for: WB
  • Reacts with: Species independent
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-GFP antibody [E385]
    See all GFP primary antibodies

  • Description

    HRP Rabbit monoclonal [E385] to GFP

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    WB
    Species independent
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK 293 over-expressing GFP lysate and Active. Pure GFP protein, or cells known to overexpress GFP.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    E385

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - HRP Anti-GFP antibody [E385] (ab190584)
    Western blot - HRP Anti-GFP antibody [E385] (ab190584)
    All lanes : HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

    Lane 1 : HEK 293 over-expressing GFP
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190485 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Western blot - HRP Anti-GFP antibody [E385] (ab190584)
    Western blot - HRP Anti-GFP antibody [E385] (ab190584)
    All lanes : HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

    Lane 1 : Recombinant A. victoria GFP protein (ab84191)
    Lane 2 : Recombinant RFP protein (ab51993)

    Lysates/proteins at 0.1 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190584 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
  • HRP Anti-GFP antibody [E385] (ab190584)
    HRP Anti-GFP antibody [E385] (ab190584)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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