Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [3H9] to GFP - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Species independent

Overview

  • Product name

    Anti-GFP antibody [3H9] - BSA and Azide free
    See all GFP primary antibodies

  • Description

    Rat monoclonal [3H9] to GFP - BSA and Azide free

  • Host species

    Rat

  • Specificity

    This mAb recognizes eGFP, wild-type GFP, YFP and CFP.

  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Other Immunogen Type. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with GFP expression vector, whole cell lysate. IHC-P: HEK-293T transfected with a GFP construct. ICC: HEK-293T cells.

  • General notes

    ab255886 is the carrier-free version of ab252881. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255886 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    3H9

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)
    Western blot - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)
    All lanes : Anti-GFP antibody [3H9] (ab252881) at 1/5000 dilution

    Lane 1 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 2 : HEK-293T transfected with GFP expression vector, whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 3 seconds


    This data was developed using ab252881 the same antibody clone in a different buffer formulation.

    Blocking and Diluting buffer: 5% NFDM/TBST

    Exposure time: 3 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using ab252881 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GFP construct (A) labeling GFP with ab252881 at 1/2000 dilution (0.539 µg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). No staining observed on HEK-293T cells (B). The section was incubated with ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. 

  • Immunocytochemistry - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)
    Immunocytochemistry - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using ab252881 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for GFP (red) using ab252881 at 1/100 dilution (10.78 μg/ml), followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in HEK-293T cells transfected with GFP only plasmid. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using ab252881 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human tonsil section labeling GFP with ab252881 at 1/2000 dilution (0.539 µg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). The section was incubated with ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Negative control: no staining on human tonsil. 

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using ab252881 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse spleen section labeling GFP with ab252881 at 1/2000 dilution (0.539 µg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). The section was incubated with ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Negative control: no staining on mouse spleen. 

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using ab252881 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat liver section labeling GFP with ab252881 at 1/2000 dilution (0.539 µg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). The section was incubated with ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Negative control: no staining on rat liver. 

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. 

  • Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)
    Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

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