Alexa Fluor® 488 Anti-GFP VHH Single Domain antibody (ab192863)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Llama monoclonal to GFP VHH Single Domain
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-GFP VHH Single Domain antibody
See all GFP VHH Single Domain primary antibodies -
Description
Alexa Fluor® 488 Llama monoclonal to GFP VHH Single Domain -
Host species
Llama -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Specificity
Specifically binds GFP, eGFP and BFP. ab192863 binds GFP N-terminal and C-terminal fusion proteins. It does not interact with CFP, RFP, YFP, mCherry or TurboGFP. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
Recombinant full length protein corresponding to Aequorea victoria GFP VHH Single Domain.
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Positive control
- This antibody gave a positive signal in NIH3T3 overexpressing GFP cell line within IP.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, Sodium chloride, Sodium phosphate, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This product is a recombinant protein produced in E. coli. -
Clonality
Monoclonal -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFP VHH Single Domain antibody (ab192863)
Anti-GFP VHH Single Domain Antibody (Alexa Fluor® 488) (ab192863) staining GFP in GFP-transfected NIH3T3 cells. The cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab192863, at 0.1μg/ml overnight at +4°C (green). Nuclear DNA was labelled with 1.43μM DAPI (blue).
Under identical experimental conditions, when compared to the basal level of GFP expression in transfected NIH3T3 cells, the cells upon which ab192863 was applied gave a stronger signal in the 488 channel, indicating that ab192863 is binding to GFP and therefore eliciting signal amplification.
Ab192863 was also applied to non-GFP-transfected NIH3T3 cells, which produced no positive staining, indicating specificity for GFP.
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Alexa Fluor® 488 Anti-GFP VHH Single Domain antibody (ab192863)
