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Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)

Key features and details

  • Rabbit polyclonal to HNF-4-alpha + HNF-4-gamma
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HNF-4-alpha + HNF-4-gamma antibody

  • Description

    Rabbit polyclonal to HNF-4-alpha + HNF-4-gamma

  • Host species

    Rabbit

  • Specificity

    Rabbit polyclonal to HNF-4-alpha + HNF-4-gamma antibody detects endogenous levels of total HNF-4-alpha/gamma protein.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human HNF-4-alpha + HNF-4-gamma (internal sequence).

  • Positive control

    • Extracts from HeLa cells, HepG2 cells and 293 cells.

Properties

Images

  • Western blot - Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)
    Western blot - Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)
    All lanes : Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HepG2 cells
    Lane 3 : extracts from 293 cells
    Lane 4 : extracts from 293 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa
    Additional bands at: 30 kDa, 40 kDa, 65 kDa, 75 kDa, 90 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)
    Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)
    ICC/IF image of ab64911 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64911, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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