Mouse Tissue Factor ELISA Kit (ab214091)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.7 pg/ml
- Range: 25 pg/ml - 1600 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Tissue Factor ELISA Kit
See all Tissue Factor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 3% Inter-assay Sample n Mean SD CV% Overall 5 3% -
Sample type
Cell culture supernatant, Urine, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
3.7 pg/ml -
Range
25 pg/ml - 1600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92 87% - 98% Urine 99 97% - 99% Cell culture extracts 119 117% - 122% Mouse brain extract 109 107% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Tissue Factor ELISA Kit (ab214091) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tissue Factor protein in cell culture extracts, cell culture supernatant, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Tissue Factor with 3.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Tissue factor plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Tissue Factor initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The tissue factor-VIIa complex activates factors IX or X by specific limited protolysis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Tissue Factor Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse Tissue Factor Capture Antibody (Lyophilized) 1 vial Mouse Tissue Factor Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. -
Sequence similarities
Belongs to the tissue factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD142
- CD142 antigen
- Coagulation factor III
see all -
Database links
- Entrez Gene: 14066 Mouse
- SwissProt: P20352 Mouse
- Unigene: 273188 Mouse
Images
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Other - Mouse Tissue Factor ELISA Kit (ab214091)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Tissue Factor standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Tissue Factor standard curve Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Tissue Factor in mouse cell and tissue extract samples based on a 75 µg/mL extract load.The concentrations of Tissue Factor were measured in duplicate and interpolated from the Tissue Factor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 713 pg/mL in Mouse Brain Tissue Extract, 206 pg/mL in mouse C2C12 Cell Extract and 824 pg/mL in NIH/3T3 Cell Extract.
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Interpolated concentrations of native Tissue Factor in mouse brain cell culture supernatant and mouse urine samples.The concentrations of Tissue Factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse brain cell culture supernatant 100%, mouse urine 100%. For mouse urine, dilutions beyond 2 fold were not analysed due to O.D. values below the O.D. values of the MDD. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 79 pg/mL in mouse brain cell culture supernatant and 11 pg/mL in mouse urine.
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Sandwich ELISA - Mouse Tissue Factor ELISA Kit (ab214091)To learn more about the advantages of recombinant antibodies see here.
