Recombinant LukD protein (ab190423)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: WB, ELISA, SDS-PAGE, Functional Studies
-
Product name
Recombinant LukD protein -
Biological activity
Cytotoxicity can be detected in Human neutrophils when used in combination with LukE in a concentration range of 12.5-800 nM.
-
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Sequence
MKIEKLGKSSVASSIALLLLSNTVDAAQNITPKREKKVDDKITLYKTTAT SDNDKLNIFQILTFNFIKDKSYDKDTLVLKAAGNINSGYKNSNPKDYNYS QFYWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINI SNGLSGGLNGSKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNN GWGPYGRDSYDPTYGNELFLGGDKSSSNAGQNFLPTHQIPLLARGNFNPE FISVLSHKLFDTKKSKIKVTYQREMDRYTNQWNRSHWVGNNYKNQNTVTF TSTYEVDWQNILLKLIGTDSKETNPGV -
Predicted molecular weight
37 kDa -
Amino acids
1 to 327 -
Additional sequence information
From Staphylococcus aureus. Purified.
-
Preparation and Storage
- Leukotoxin LukD
Images
-
SDS-PAGE - Recombinant LukD protein (ab190423)
SDS-PAGE analysis of ab190423: 1 µg (lane 1) and 5 µg (lane 2).
-
Western blot - Recombinant LukD protein (ab190423)Anti-LukF-PV polyclonal antibody at 0.5 µg/ml +Recombinant LukD protein (ab190423) at 0.1 µg
Secondary
Anti-rabbit IgG-HRP conjugate -
Functional Studies - Recombinant LukD protein (ab190423)Toxin Functionality: Human promyelocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of rLukE and ab190423 alone (Open circles and open squares, respectively) or equimolar concentration of both proteins (Closed circles) for 3 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470nm. EC50 was found to be 95.1 nM of each toxin subunit.
