Recombinant LukE protein (ab190128)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: ELISA, WB, SDS-PAGE, Functional Studies
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Product name
Recombinant LukE protein -
Biological activity
Cytotoxicity can be detected in human neutrophils when used in combination with LukD in a concentration range of 12.5-800 nM.
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Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Sequence
NTNIENIGDGAEVIKRTEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKM QGFINSRTSF SDVKGRGYELTKRLIWPFQYNIGLTTKDPNVSLINSIT LPKTKIETTDVGQTLGYNIGGN FQSAPSIGGNGSFNYSKTISYTQKSY VSEVDKQNSKSVKWGVKANKFVTPDGKKFAHDRY LFVQSPNGPTGSAR EYFAPDNQLPPLVQSGFNPSFITTLSHEKGSKLIRVNLKFSYGRNL DI TYATLFPRTGIYAERKHNAFVNRNFVVRYKVNWKTHEIKVKGHN -
Predicted molecular weight
35 kDa -
Amino acids
29 to 314 -
Additional sequence information
Mature LukE toxin corresponding to amino acids 29-314 of LukE from Staphylococcus aureus.
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Preparation and Storage
- Leucotoxin LukE
Images
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Western blot - Recombinant LukE protein (ab190128)rabbit polyclonal antibody anti LukE at 0.5 µg/ml +
Recombinant LukE protein (ab190128) at 0.1 µg
Secondary
anti rabbit IgG HRP conjugated followed by substrate -
SDS-PAGE - Recombinant LukE protein (ab190128)SDS PAGE analysis of ab190128 at 1 μg/ml (lane 1) and 5 μg/ml (lane 2).
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Functional Studies - Recombinant LukE protein (ab190128)Cytotoxicity assay in HL60 cells differentiated into neutrophils after DMSO treatment. Open circles curve represents toxin functionality of ab190128 at serial dilutions.