Human Tissue Factor ELISA Kit (CD142) (ab108903)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 12.5 pg/ml - 100 pg/ml
- Sample type: Cell culture extracts, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Tissue Factor ELISA Kit (CD142)
See all Tissue Factor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.1% Inter-assay Sample n Mean SD CV% Overall 9.8% -
Sample type
Serum, Plasma, Cell culture extracts, Tissue -
Assay type
Sandwich (quantitative) -
Sensitivity
= 20 pg/ml -
Range
12.5 pg/ml - 100 pg/ml -
Recovery
97 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Tissue Factor (CD142) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor concentrations in plasma, serum, tissue extracts and cell culture supernatants.
A Tissue Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Tissue Factor Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Tissue Factor Microplate (12 x 8 well strips) 1 unit Tissue Factor Standard 1 vial -
Research areas
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Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. -
Sequence similarities
Belongs to the tissue factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD142
- CD142 antigen
- Coagulation factor III
see all -
Database links
- Entrez Gene: 2152 Human
- Omim: 134390 Human
- SwissProt: P13726 Human
- Unigene: 62192 Human
Images
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Sandwich ELISA - 108903 Tissue Factor (CD142) Human ELISA Kit
CD142 measured in cell lysates showing quantity (pg) per 1 million cells tested
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Sandwich ELISA - 108903 Tissue Factor (CD142) Human ELISA KitCD142 measured in various biofluids showing quantity (pg) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab108903.
