Human HNF-4-alpha Matched Antibody Pair Kit (ab218176)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 31.25 pg/ml - 2000 pg/ml
Overview
-
Product name
Human HNF-4-alpha Matched Antibody Pair Kit
See all HNF-4-alpha kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.05 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human HNF-4-alpha Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human HNF-4-alpha.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210581. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human HNF-4 alpha Lyophilized Protein 2 vials 1 vial Human HNF-4 alpha Capture Antibody 2 x 50µg 1 x 50µg Human HNF-4 alpha Detector Antibody 2 x 12.5µg 1 x 12.5µg -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
-
Function
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. -
Involvement in disease
Defects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Post-translational
modificationsPhosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. -
Cellular localization
Nucleus. - Information by UniProt
-
Alternative names
- FLJ39654
- FRTS4
- Hepatic nuclear factor 4 alpha
see all -
Database links
- Entrez Gene: 3172 Human
- Omim: 600281 Human
- SwissProt: P41235 Human
- Unigene: 116462 Human
Images
-
Human HNF-4-alpha standard curve.
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Human HNF-4-alpha Matched Antibody Pair Kit (ab218176)To learn more about the advantages of recombinant antibodies see here.
