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Cell Biology Proteolysis / Ubiquitin Protease inhibitors

Anti-Cystatin-B antibody [2F1] (ab72353)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Cystatin-B antibody [2F1] (ab72353)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [2F1] to Cystatin-B
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Cystatin-B antibody [2F1]
    See all Cystatin-B primary antibodies
  • Description

    Mouse monoclonal [2F1] to Cystatin-B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Cystatin-B.

  • Positive control

    • A549 cell lysate
  • General notes

    Cystatin-B is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cystatin-B protein.

    This product was previously labelled as Stefin B.

    This product was changed from ascites to tissue culture supernatant on 08/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    2F1
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Other protease inhibitors

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cystatin-B antibody [2F1] (ab72353)
    Immunocytochemistry/ Immunofluorescence - Anti-Cystatin-B antibody [2F1] (ab72353)

    ICC/IF analysis of CSTB in A549 cells. The cell was stained with ab72353(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

  • Western blot - Anti-Cystatin-B antibody [2F1] (ab72353)
    Western blot - Anti-Cystatin-B antibody [2F1] (ab72353)
    All lanes : Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : U87MG cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 11 kDa

  • Western blot - Anti-Cystatin-B antibody [2F1] (ab72353)
    Western blot - Anti-Cystatin-B antibody [2F1] (ab72353)
    Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution + A549 cells lysate at 35 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: IHC-P, WB

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    Anti-Cystatin-B antibody (ab236646)

    Applications: ICC/IF, IHC-P, IP, WB

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