Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cystatin-B antibody [EPR3931] (ab92449)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3931] to Cystatin-B
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Cystatin-B antibody [EPR3931]
    See all Cystatin-B primary antibodies

  • Description

    Rabbit monoclonal [EPR3931] to Cystatin-B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cystatin-B aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, U87-MG, JAR, and Human bladder cell lysates. IHC-P: Human colon tissue, human kidney tissue.

  • General notes

    We have preliminary internal testing data to indicate this Cystatin-B antibody (ab92449) may not react with mouse. Please contact us for more information. ab92449 was previously labelled as Stefin B

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cystatin-B antibody [EPR3931] (ab92449)
    Western blot - Anti-Cystatin-B antibody [EPR3931] (ab92449)
    All lanes : Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : CSTB knockout HEK293T cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 11 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab92449 observed at 15 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab92449 Anti-Cystatin-B antibody [EPR3931] was shown to specifically react with Cystatin-B in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266321 (knockout cell lysate ab257905) was used. Wild-type and Cystatin-B knockout samples were subjected to SDS-PAGE. ab92449 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] (ab92449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] (ab92449)

    ab92449, at 1/100 dilution, staining Cystatin-B in paraffin-embedded Human colon tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Western blot - Anti-Cystatin-B antibody [EPR3931] (ab92449)
    Western blot - Anti-Cystatin-B antibody [EPR3931] (ab92449)
    All lanes : Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : Human bladder lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] (ab92449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] (ab92449)

    ab92449, at 1/100 dilution, staining Cystatin-B in paraffin-embedded Human kidney tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-Cystatin-B antibody [EPR3931] (ab92449)
    Anti-Cystatin-B antibody [EPR3931] (ab92449)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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