Anti-Tissue Factor antibody [EPR18160-175] (ab189483)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18160-175] to Tissue Factor
- Suitable for: IP, WB
- Reacts with: Mouse
Overview
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Product name
Anti-Tissue Factor antibody [EPR18160-175]
See all Tissue Factor primary antibodies -
Description
Rabbit monoclonal [EPR18160-175] to Tissue Factor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse skin and brain tissue lysate; NIH/3T3 whole cell lysate. IP: Mouse brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18160-175 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Tissue Factor antibody [EPR18160-175] (ab189483) at 1/1000 dilution
Lane 1 : Mouse skin tissue lysate
Lane 2 : Mouse brain tissue lysate
Lanes 3-4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33, 47 kDa why is the actual band size different from the predicted?Blocking/Dilution: 5% NFDM/TBST.
Exposure: Lanes 1 and 3: 3 minutes; Lanes 2 and 4: 10 seconds.
The observed molecular mass is consistent with the literature (PMID: 7992837).
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Tissue Factor was immunoprecipitated from 0.35 mg of mouse brain lysate with ab189483 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189483 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate 10 µg (Input).
Lane 2: ab189483 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab1189483 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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