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Cardiovascular Blood Coagulation Extrinsic

Anti-Tissue Factor antibody [EPR18160-175] (ab189483)

Price and availability

278 083 ₸

Availability

Order now and get it on Friday March 05, 2021

Anti-Tissue Factor antibody [EPR18160-175] (ab189483)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18160-175] to Tissue Factor
  • Suitable for: IP, WB
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-Tissue Factor antibody [EPR18160-175]
    See all Tissue Factor primary antibodies
  • Description

    Rabbit monoclonal [EPR18160-175] to Tissue Factor
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse skin and brain tissue lysate; NIH/3T3 whole cell lysate. IP: Mouse brain tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18160-175
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Coagulation
    • Extrinsic
    • Cardiovascular
    • Blood
    • Coagulation
    • Common
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • CD markers ELISA kits

Images

  • Western blot - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)
    Western blot - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)
    All lanes : Anti-Tissue Factor antibody [EPR18160-175] (ab189483) at 1/1000 dilution

    Lane 1 : Mouse skin tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lanes 3-4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33, 47 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution: 5% NFDM/TBST.

    Exposure: Lanes 1 and 3: 3 minutes; Lanes 2 and 4: 10 seconds.

    The observed molecular mass is consistent with the literature (PMID: 7992837).

  • Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)
    Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)

    Tissue Factor was immunoprecipitated from 0.35 mg of mouse brain lysate with ab189483 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189483 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain lysate 10 µg (Input). 

    Lane 2: ab189483 IP in mouse brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab1189483 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • Anti-Tissue Factor antibody [EPR18160-175] (ab189483)
    Anti-Tissue Factor antibody [EPR18160-175] (ab189483)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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