Anti-Tissue Factor antibody [MM0700-21U4] (ab201598)
![Anti-Tissue Factor antibody [MM0700-21U4] (ab201598)](https://www.abcam.com/ps/products/201/ab201598/Images/ab201598-304233-anti-tissue-factor-antibody-mm0700-21u4-western-blot.jpg "Anti-Tissue Factor antibody [MM0700-21U4] (ab201598)")
Key features and details
- Mouse monoclonal [MM0700-21U4] to Tissue Factor
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2
Overview
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Product name
Anti-Tissue Factor antibody [MM0700-21U4]
See all Tissue Factor primary antibodies -
Description
Mouse monoclonal [MM0700-21U4] to Tissue Factor -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human Tissue Factor aa 1-250.
Database link: P13726
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MM0700-21U4 -
Isotype
IgG2 -
Research areas
Images
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Western blot - Anti-Tissue Factor antibody [MM0700-21U4] (ab201598)
Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: Tissue Factor knockout HAP1 whole cell lysate (40 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab201598 observed at 49 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab201598 was shown to recognize Tissue Factor in wild-type HAP1 cells as signal was lost at the expected MW in Tissue Factor knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Tissue Factor knockout samples were subjected to SDS-PAGE. Ab201598 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
