Anti-Tissue Factor antibody [EPR20369] - BSA and Azide free (ab242432)
![Anti-Tissue Factor antibody [EPR20369] - BSA and Azide free (ab242432)](https://www.abcam.com/ps/products/242/ab242432/Images/ab242432-332204-ab211016304504211016KOWBMPES.jpg "Anti-Tissue Factor antibody [EPR20369] - BSA and Azide free (ab242432)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20369] to Tissue Factor - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Tissue Factor antibody [EPR20369] - BSA and Azide free
See all Tissue Factor primary antibodies -
Description
Rabbit monoclonal [EPR20369] to Tissue Factor - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human placenta. kidney and pancreas cancer tissues.
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General notes
Ab242432 is the carrier-free version of ab211016. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab242432 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: 59% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20369 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tissue Factor antibody [EPR20369] (ab242432)All lanes : Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Tissue Factor knockout HAP1 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 33 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab211016 observed at 49 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab211016 was shown to recognize Tissue Factor in wild-type HAP1 cells as signal was lost at the expected MW in Tissue Factor knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and F3 (Tissue Factor) knockout samples were subjected to SDS-PAGE. Ab211016 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211016).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab242432)](https://www.abcam.com/ps/products/242/ab242432/Images/ab242432-3-ab211016272178211016IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab242432)Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Tissue Factor with ab211016 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.
Cytoplasmic staining on human placenta is observed [PMID: 2237820].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211016).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab242432)](https://www.abcam.com/ps/products/242/ab242432/Images/ab242432-2-ab211016272176211016IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab242432)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tissue Factor with ab211016 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.
Cytoplasmic staining on human kidney glomerulus is observed [PMID: 2237820].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211016)
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab242432)](https://www.abcam.com/ps/products/242/ab242432/Images/ab242432-1-ab211016272175211016IHCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab242432)Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling Tissue Factor with ab211016 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.
Membranous and cytoplasmic staining on tumor cells of human pancreas cancer is observed [PMID: 7648165].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211016).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Tissue Factor antibody [EPR20369] - BSA and Azide free (ab242432)](https://www.abcam.com/ps/products/242/ab242432/Images/ab242432-5-benefits-of-recombinant-antibodies.png)
Anti-Tissue Factor antibody [EPR20369] - BSA and Azide free (ab242432)
