Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)
![Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)](https://www.abcam.com/ps/products/251/ab251275/Images/ab251275-408158-anti-hnf-4-alpha-antibody-epr16786-bsa-and-azide-free-western-blot.jpg "Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16786] to HNF-4-alpha - BSA and Azide free
- Suitable for: WB, ICC, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free
See all HNF-4-alpha primary antibodies -
Description
Rabbit monoclonal [EPR16786] to HNF-4-alpha - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251275 is the carrier-free version of ab199431. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251275 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16786 -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
Images
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Western blot - Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)All lanes : Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab199431) at 1/10000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : Human fetal liver tissue lysate
Lane 4 : Human stomach tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 47, 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab199431, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
This antibody can recognize 3 isoforms. The predicted MW are 53KDa, 52 KDa and 47KDa, respectively.
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![Western blot - Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)](https://www.abcam.com/ps/products/251/ab251275/Images/ab251275-405828-anti-hnf-4-alpha-antibody-epr16786-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab199431) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 47, 53 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis data was developed using ab199431, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
This antibody can recognize 3 isoforms. The predicted MW are 53KDa, 52 KDa and 47KDa, respectively.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)](https://www.abcam.com/ps/products/251/ab251275/Images/ab251275-2-anti-hnf-4-alpha-antibody-epr16786-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)This data was developed using ab199431, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling HNF-4-alpha with ab199431 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)](https://www.abcam.com/ps/products/251/ab251275/Images/ab251275-1-anti-hnf-4-alpha-antibody-epr16786-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)This data was developed using ab199431, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HNF-4-alpha with ab199431 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab199431 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)](https://www.abcam.com/ps/products/251/ab251275/Images/ab251275-5-benefits-of-recombinant-antibodies.png)
Anti-HNF-4-alpha antibody [EPR16786] - BSA and Azide free (ab251275)