Anti-HNF-4-alpha antibody (ab36175)
Key features and details
- Goat polyclonal to HNF-4-alpha
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-HNF-4-alpha antibody
See all HNF-4-alpha primary antibodies -
Description
Goat polyclonal to HNF-4-alpha -
Host species
Goat -
Specificity
This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.). -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
-
Immunogen
Synthetic peptide corresponding to Human HNF-4-alpha aa 2-15 (N terminal).
Sequence:RLSKTLVDMDMADY
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
Images
-
Western blot - Anti-HNF-4-alpha antibody (ab36175)Anti-HNF-4-alpha antibody (ab36175) at 0.5 µg/ml + Mouse Small Intestine tissue lysate at 35 µg
Predicted band size: 50 kDa
Observed band size: 55, 48 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
-
Western blot - Anti-HNF-4-alpha antibody (ab36175)Anti-HNF-4-alpha antibody (ab36175) at 0.1 µg/ml + HepG2 lysate (35µg protein in RIPA buffer).
Predicted band size: 50 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
