Human Tissue Factor ELISA Kit (ab220653)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.6 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Tissue Factor ELISA Kit
See all Tissue Factor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 4.9% Inter-assay Sample n Mean SD CV% Overall 3 6.9% -
Sample type
Cell culture supernatant, Saliva, Urine, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.6 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 105% - 116% Saliva 86 83% - 87% Urine 83 80% - 85% Cell culture extracts 105 100% - 107% Hep Plasma 109 107% - 113% EDTA Plasma 115 111% - 118% Cit plasma 104 99% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Tissue Factor ELISA Kit (ab220653) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tissue Factor protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tissue Factor with 3.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Tissue factor (TF), also known as Coagulation factor III, F3 thromboplastin, and CD142 is a 263-amino acid single pass type I membrane protein encoded by the gene F3. TF is a 47 kDa single pass type I membrane protein consisting of an extracellular, transmembrane, and cytoplasmic domain. Tissue factor’s main role is in blood coagulation. The protein acts as a receptor for coagulation factor VII, which then forms the active complex FVIIa. This complex proteolytically activates downstream factors, including coagulation Factor IX and X, which leads to the formation of thrombin and fibrin clot formation. Tissue Factor is in a class of proteins known as Cytokine Receptor class II family. This family is activated by cytokines, and play a role in angiogenesis and apoptosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Tissue Factor Capture Antibody 1 x 600ml 10X Human Tissue Factor Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Tissue Factor Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. -
Sequence similarities
Belongs to the tissue factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD142
- CD142 antigen
- Coagulation factor III
see all -
Database links
- Entrez Gene: 2152 Human
- Omim: 134390 Human
- SwissProt: P13726 Human
- Unigene: 62192 Human
Images
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Other - Human Tissue Factor ELISA Kit (ab220653)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Tissue Factor standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Tissue Factor standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Tissue Factor in human plasma and A431 cell culture supernatant samples.The concentrations of Tissue factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, saliva 50% and A431 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 50 pg/mL in plasma (citrate), 78 pg/mL in plasma (heparin), 65.7 pg/mL in plasma (EDTA), 98 pg/mL in saliva) and 97.5 pg/mL in A431 cell culture supernatant.
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Interpolated concentrations of spiked Tissue Factor in human plasma, saliva, and urine samples.The concentrations of Tissue Factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: 50%, plasma (citrate) 50%, plasma (heparin) 50%, saliva 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 1,000 pg/mL in neat plasma (citrate), plasma (heparin), plasma (EDTA), saliva and 2,000 pg/mL in neat urine.
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Interpolated concentrations of native Tissue Factor in U-87 and A431 cellular extract samples based on a 1,000 µg/mL extract load.The concentrations of Tissue Factor were measured in duplicate and interpolated from the Tissue Factor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 10.4 ng/mL in U-87 cell extract and 100 ng/mL in A431 cell extract.
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Sandwich ELISA - Human Tissue Factor ELISA Kit (ab220653)To learn more about the advantages of recombinant antibodies see here.
