HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)
![HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)](https://www.abcam.com/ps/products/209/ab209473/Images/ab209473-354270-WBab2094732.jpg "HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR16786] to HNF-4-alpha - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal
See all HNF-4-alpha primary antibodies -
Description
HRP Rabbit monoclonal [EPR16786] to HNF-4-alpha - N-terminal -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 whole cell and Mouse Liver tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR16786 -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
Images
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Western blot - HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)All lanes : HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473) at 1/1000 dilution
Lane 1 : HepG2 Whole Cell Lysate
Lane 2 : Mouse Liver Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 47,53 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk/1x TBST blocking buffer before being incubated with ab209473 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Western blot - HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)](https://www.abcam.com/ps/products/209/ab209473/Images/ab209473-282228-anti-hnf-4-alpha-antibody-epr16786-n-terminal-hrp-western-blot.jpg)
Western blot - HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)All lanes : HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 47,53 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209473 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)](https://www.abcam.com/ps/products/209/ab209473/Images/ab209473-3-benefits-of-recombinant-antibodies.png)
HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)
