Human Tissue Factor Matched Antibody Pair Kit (ab221438)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Human Tissue Factor Matched Antibody Pair Kit
See all Tissue Factor kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Tissue Factor Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Tisue Factor.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab220653. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Tissue Factor Capture Antibody 2 x 50µg 1 x 50µg Human Tissue Factor Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Tissue Factor Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. -
Sequence similarities
Belongs to the tissue factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD142
- CD142 antigen
- Coagulation factor III
see all -
Database links
- Entrez Gene: 2152 Human
- Omim: 134390 Human
- SwissProt: P13726 Human
- Unigene: 62192 Human
Images
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Human tissue factor standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Tissue Factor Matched Antibody Pair Kit (ab221438)To learn more about the advantages of recombinant antibodies see here.
