Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free (ab254007)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22548-240] to Tissue Factor - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free
    See all Tissue Factor primary antibodies

  • Description

    Rabbit monoclonal [EPR22548-240] to Tissue Factor - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, A431, MDA-MB-231 and BxPC-3 whole cell lysates. IHC-P: Human kidney and cervix carcinoma tissue.

  • General notes

    Ab254007 is the carrier-free version of ab252918. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254007 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free (ab254007)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free (ab254007)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on tumor cells of human cervix carcinoma (PMID: 26383146) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab252918 for 30 minutes at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252918).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free (ab254007)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free (ab254007)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human renal glomerulus (PMID: 7684196) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab252918 for 30 minutess at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252918).

  • Western blot - Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free (ab254007)
    Western blot - Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free (ab254007)
    All lanes : Anti-Tissue Factor antibody [EPR22548-240] (ab252918) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate, 60 ug
    Lane 2 : Tissue Factor knockout HAP1 whole cell lysate, 60 ug
    Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate, 20 ug
    Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate, 20 ug
    Lane 5 : BxPC-3 (human pancreas adenocarcinoma epithelial cell line) whole cell lysate, 20 ug

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa



    Blocking and dilution buffer:5% NFDM/TBST.

    Exposure times.
    Lanes 1 2: 70 seconds. Lanes 3-6: 3.25 seconds.

    ab252918 was shown to specifically react with Tissue Factor in wild-type HAP1 cells as signal was lost in Tissue Factor knockout cells. Wild-type and Tissue Factor knockout samples were subjected to SDS-PAGE. ab252918 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

    The blot was developed on a BIO-RAD ChemiDoc MP instrument using the ECL technique. The molecular weight observed is consistent with what has been described in the literature (PMID: 28938620). Low expression:MCF7 (PMID: 24137414, 28938620).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252918).

     

  • Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free (ab254007)
    Anti-Tissue Factor antibody [EPR22548-240] - BSA and Azide free (ab254007)

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