Recombinant A. victoria GFP protein (ab84191)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.316% Tris HCl, 10% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant A. victoria GFP protein (ab84191)
1. Prepare a 150 μl recombinant GFP with various concentrations (0.00074 nM - 6.21 μM) in assay buffer and equilibrate to 25°C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide.)
2. Read at excitation wavelengths 485 nm and emission 535 nm.
- 96 Well Polystyrene Microplate, black
- Fluorescent plate reader
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SDS-PAGE - Recombinant A. victoria GFP protein (ab84191)3 ug of reduced ab84191 on SDS-PAGE, stained with Coomassie Blue.
