Anti-GFP antibody (ab5449)
Key features and details
- Goat polyclonal to GFP
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-GFP antibody
See all GFP primary antibodies -
Description
Goat polyclonal to GFP -
Host species
Goat -
Specificity
Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP. -
Tested Applications & Species
See all applications and species dataApplication Species WB Species independent
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Immunogen
This information is considered to be commercially sensitive.
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Positive control
- Pure GFP protein, or cells known to overexpress GFP.
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General notes
Protein A will not bind goat IgG, so use alternates (eg. protein G) in IP with this antibody. This antibody is available in an affinity purified form as ab5450.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GFP antibody (ab5449)
Lane 1 : parental YTS cells (negative control)
Lanes 2-5 : YTS cells transfected with KIR-EGFP (mw 88 kD)KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x106 cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33: 1084).
Lane 1 : parental YTS cells (negative control) Lanes 2-5 : YTS cells transfected with KIR-EGFP (mw 88 kD) KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x106 cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33: 1084). -
Western blot - Anti-GFP antibody (ab5449)5 ng GFP on PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of:
Lane 1 : 1/2500
Lane 2 : 1/5000
Lane 3 : 1/10,000
Lane 4 : 1/20,000
5 ng GFP on PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of: Lane 1 : 1/2500 Lane 2 : 1/5000 Lane 3 : 1/10,000 Lane 4 : 1/20,000
