Alkaline Phosphatase Anti-GFP antibody (ab6661)
Key features and details
- Alkaline Phosphatase Goat polyclonal to GFP
- Suitable for: WB, ELISA, Dot blot
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Overview
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Product name
Alkaline Phosphatase Anti-GFP antibody
See all GFP primary antibodies -
Description
Alkaline Phosphatase Goat polyclonal to GFP -
Host species
Goat -
Conjugation
Alkaline Phosphatase -
Specificity
Antibody recognizes wild type, recombinant and enhanced forms of GFP. Immunoblot shows a 42 kDa band when reacted with GFP on a Western blot. No reaction was observed against human, mouse and rat serum proteins. -
Tested applications
Suitable for: WB, ELISA, Dot blotmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Fusion protein corresponding to GFP aa 1-246.
Database link: P42212 -
General notes
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat serum, anti-Alkaline Phosphatase and purified and partially purified GFP (Aequorea victoria) serum. -
Primary antibody notes
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)Alkaline Phosphatase Anti-GFP antibody (ab6661) at 1/1000 dilution + GFP at 0.05 µg with Blocking buffer
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Dot Blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)Dot blott analysis of ab6661 used in all lanes at a concentration of 1/1,000 incubated at room temperature for 1 hour.
Lane 1: 100 ng GFP protein
Lane 2: 33.33 ng GFP protein
Lane 3: 11.11 ng GFP protein
Lane 4: 3.70 ng GFP protein
Lane 5: 1.23 ng GFP protein
