Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17362] to GATA1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, ChIP, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GATA1 antibody [EPR17362] - BSA and Azide free
    See all GATA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17362] to GATA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, ChIP, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon and cervix carcinoma tissues. ICC/IF: K562 cells. IP: K562 whole cell extract. ChIP: Chromatin from K562 cells.

  • General notes

    Ab241393 is the carrier-free version of ab181544. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab241393 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
    Immunoprecipitation - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

    GATA1 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab181544 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab181544 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: K562 whole cell extract 10 µg (Input).
    Lane 2: ab181544 IP in K562 whole cell extract.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181544 in K562 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181544).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling GATA1 with ab181544 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on leukocyte of Human cervical cancer is observed. Counterstained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181544).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GATA1 with ab181544 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on leukocyte of Human colon stroma is observed. Counterstained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181544).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • ChIP - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
    ChIP - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

    Chromatin was prepared from K562 (Human chronic myelogenous leukemia cells from bone marrow) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab181544 (blue), and 20µl of Anti rabbit IgG sepharose beads. 5μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    “pro” stands for promoter region, while “NC2” stands for negative control which is negative loci at the promoter region.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181544).
  • Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
    Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

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