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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-BUD31 antibody (ab237493)

Anti-BUD31 antibody (ab237493)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to BUD31
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BUD31 antibody
  • Description

    Rabbit polyclonal to BUD31
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Zebrafish
  • Immunogen

    Recombinant full length protein corresponding to Human BUD31 aa 1 to the C-terminus.
    Database link: P41223

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Jurkat, Raji, NIH/3T3, K562, HepG2 and U-251 MG whole cell lysate; Mouse liver lysate. IHC-P: Human colon cancer and spleen tissues. ICC/IF: MCF7 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Western blot - Anti-BUD31 antibody (ab237493)
    Western blot - Anti-BUD31 antibody (ab237493)
    All lanes : Anti-BUD31 antibody (ab237493) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
    Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 7 : U-251 MG (human brain glioma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (ab237493)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (ab237493)

    Paraffin-embedded human colon cancer tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (ab237493)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (ab237493)

    Paraffin-embedded human spleen tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-BUD31 antibody (ab237493)
    Immunocytochemistry/ Immunofluorescence - Anti-BUD31 antibody (ab237493)

    MCF7 (human breast adenocarcinoma cell line) cells stained for BUD31 (green) using ab237493 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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