Anti-ZNF471/ERP1 antibody (ab204974)
Key features and details
- Rabbit polyclonal to ZNF471/ERP1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNF471/ERP1 antibody -
Description
Rabbit polyclonal to ZNF471/ERP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ZNF471/ERP1 aa 50-150.
Database link: Q9BX82 -
Positive control
- WB: RT-4 and U-251 MG whole cell lysates; IHC-P: Human colon, testis and cerebral cortex tissue; ICC: U-2 OS whole cells.
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General notes
This product was previously labelled as ZNF471
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (ab204974)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ZNF471/ERP1 in the cytoplasm and nucleus of glandular cells with ab204974 at a 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-ZNF471/ERP1 antibody (ab204974)All lanes : Anti-ZNF471/ERP1 antibody (ab204974) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 73 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-ZNF471/ERP1 antibody (ab204974)Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling ZNF471/ERP1 in the nucleus with ab204974 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (ab204974)Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue labeling ZNF471/ERP1 with ab204974 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (ab204974)Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue labeling ZNF471/ERP1 with ab204974 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (ab204974)Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue labeling ZNF471/ERP1 with ab204974 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol. No positivity as expected.