Anti-SAT1 antibody (ab105220)
Key features and details
- Rabbit polyclonal to SAT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SAT1 antibody
See all SAT1 primary antibodies -
Description
Rabbit polyclonal to SAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Macaque monkey, Gorilla, Chinese hamster
-
Immunogen
Synthetic peptide corresponding to Human SAT1 aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab127967) -
Positive control
- Recombinant Human SAT1 protein (ab87666) can be used as a positive control in WB. This antibody gave a positive signal in Human Adipose tissue lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab105220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Target
-
Function
Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine. -
Pathway
Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1. -
Involvement in disease
Keratosis follicularis spinulosa decalvans X-linked -
Sequence similarities
Belongs to the acetyltransferase family.
Contains 1 N-acetyltransferase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 100689427 Chinese hamster
- Entrez Gene: 508861 Cow
- Entrez Gene: 6303 Human
- Entrez Gene: 20229 Mouse
- Entrez Gene: 397645 Pig
- Entrez Gene: 302642 Rat
- Omim: 313020 Human
- SwissProt: Q9JHW6 Chinese hamster
see all -
Alternative names
- DC21 antibody
- Diamine acetyltransferase 1 antibody
- Diamine N acetyltransferase 1 antibody
see all
Images
-
Western blot - Anti-SAT1 antibody (ab105220)Anti-SAT1 antibody (ab105220) at 1 µg/ml + Human adipose normal tissue lysate - total protein (ab28980) at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Additional bands at: 110 kDa, 39 kDa, 60 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Datasheets and documents
References (2)
ab105220 has been referenced in 2 publications.
- Tamari K et al. Polyamine flux suppresses histone lysine demethylases and enhances ID1 expression in cancer stem cells. Cell Death Discov 4:104 (2018). PubMed: 30455990
- Li X et al. LC-MS-based metabolomics revealed SLC25A22 as an essential regulator of aspartate-derived amino acids and polyamines inKRAS-mutant colorectal cancer. Oncotarget 8:101333-101344 (2017). PubMed: 29254168
Images
-
Western blot - Anti-SAT1 antibody (ab105220)Anti-SAT1 antibody (ab105220) at 1 µg/ml + Human adipose normal tissue lysate - total protein (ab28980) at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Additional bands at: 110 kDa, 39 kDa, 60 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
