Anti-GATA1 (phospho S142) antibody (ab28816)
Key features and details
- Rabbit polyclonal to GATA1 (phospho S142)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GATA1 (phospho S142) antibody
See all GATA1 primary antibodies -
Description
Rabbit polyclonal to GATA1 (phospho S142) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 142.
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Positive control
- k562 cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
After immunogen affinity purification the antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 (phospho S142) antibody (ab28816)ab28816 at (1:50-1:100), staining paraffin embedded human breast carcinoma. Left: Using GATA1 antibody (ab28816); Right: antibody preincubated with synthesized phosphopeptide.
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Western blot - Anti-GATA1 (phospho S142) antibody (ab28816)Lane 1 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution
Lane 2 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution (preincubated with synthesized non-phosphopeptide)
Lane 3 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution (preincubated with synthesized phosphopeptide)
All lanes : K562 cells
Observed band size: 43 kDa why is the actual band size different from the predicted?
